Adsorption of cytochrome c to phospholipid monolayers studied by reflection spectroscopy
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چکیده
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The adsorption of prothrombin to phospholipid monolayers quantitated by ellipsometry.
We investigated by means of an automated ellipsometer the calcium-dependent binding of prothrombin from a buffer solution to monolayers of dioleoylphosphatidylserine (DOPS) and dioleoylphosphatidylcholine (DOPC) deposited on chromium slides. This technique allows direct measurements of bound and free protein concentrations and is not hampered by calcium-induced aggregation of vesicles. For pure...
متن کاملPhospholipid–cytochrome c interactions
9 REVIEW OF THE LITERATURE 11 Structure of cytochrome c . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Phospholipid binding by cyt c . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 Cyt c in apoptosis . ....
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imidazole, which functions as an axial ligand for Mn'". This system was used to catalyze the epoxidation of water-soluble (2,5-dihydrofuran, 3) and -insoluble (styrene, 4) alkene^.^ The reactions followed first-order kinetics. GCMS and comparison with authentic samples showed that for 3 the reaction products are 3,4-epoxytetrahydrofuran and the ring-opened compound trans-3,4-dihydroxytetrahydro...
متن کاملMolecular conformation of DPPC phospholipid Langmuir and Langmuir-Blodgett monolayers studied by heterodyne-detected vibrational sum frequency generation spectroscopy.
Heterodyne-detected (phase-sensitive) vibrational sum frequency generation spectroscopy was used to investigate molecular structures of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers on water (Langmuir monolayer) and monolayers on a fused silica substrate (Langmuir-Blodgett [LB] monolayer). The spectral features in the CH stretching region depended on the phase of the Langmuir mo...
متن کاملA cytochrome c-phospholipid complex.
Lipids may play an important role in the maintenance of proper spatial configuration between the various members of the mitochondrial electron transport chain (1, 2). Greenlees and Wainio (3) and Hatefi (4) have shown that phospholipids are essential for the activity of cytochrome c oxidase. In its present state of purity, thii enzyme contains approximately 15% phospholipid and may exist as a l...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1988
ISSN: 0014-5793
DOI: 10.1016/0014-5793(88)81159-1